1990: Degree in Biological Sciences, University of Padova (Italy)

1991-1994: Graduate Fellow, Dept. of Biomedical Sciences, University of Padova (Italy)

1995-1998: PhD in “Sperimental Physiophatology” at the University of Pavia (Italy)

1998-2001: E.U. fellowship on laboratory of Prof. M.C. Sorgato, Dept. of Biological Chemistry, University of Padova (Italy)

2002-present: Research Fellow, Neuroscience Institute of Italian Research Council (CNR)

The initial research projects of Dr. Massimino focused on growth, maintenance and differentiation of primary cell cultures of different type (lymphocytes, macrophages, bone marrow stem cell, muscle cells). Her efforts than concentrated on skeletal muscle regeneration with particular regard to satellite cell activation and apoptosis. At this purpose she co-cultured satellite cells and activated macrophages in an in vitro experimental model of muscle regeneration. Her interests then shifted to transfection studies on skeletal muscle cell either in vitro or in vivo.

During last years Dr. Massimino has been involved in research projects concerning neurodegenerative disorders in particular physiopathology of the prion protein (PrP) in the neuronal cell culture (primary astrocytes, neurons and myocytes) and in vivo models (wild type and transgenic-PrP mice).

  1. V. Robert, M.L. Massimino, V. Tosello, R. Marsault, M. Cantini, V. Sorrentino, T. Pozzan. Alteration in calcium handling at the subcellular level in mdx myotubes. J. Biol. Chem., 276, 4647-4651, 2001.
  2. A. Negro, C. Ballarin, A. Bertoli, M.L. Massimino, M.C. Sorgato. The metabolism and imaging in live cells of the bovine prion protein in the native form or carrying single amino acid substitutions. Mol. Cell. Neurosci.,17, 521-538, 2001.
  3. A. Nori, G. Valle , M.L. Massimino, P. Volpe P. Targeting of Calsequestrin to the sarcoplasmic reticulum of skeletal muscle upon deletion of its glycosilation site. Exp. Cell Res, 265, 104-13, 2001.
  4. M.L. Massimino, C. Griffoni, E. Spisni, M. Toni, V. Tomasi. Involvement of caveolae and caveolae-like domains in signalling, cell survival and angiogenesis. Cell Signal., 2, 93-8, 2002.
  5. P. Ruzza, A. Donella-Deana, A. Calderan, A. Brunati, M.L. Massimino, S.Elardo, A. Mattiazzo, L.A. Pinna, G. Borin. Antennapedia/HS1 chimeric phosphotyrosyl peptide: conformational properties, binding capability to c-Fgr SH2 domain and cell permeability. Biopolymers. 4, 290-306, 2001.
  6. A. Negro, A.M. Brunati, A. Donella-Deana, M.L. Massimino, L.A. Pinna. Multiple phosphorylation of alpha-synuclein by protein tyrosine kinase Syk prevents eosin-induced aggregation. FASEB J. 2, 210-2, 2002.
  7. M.L. Massimino, C. Ballarin, A. Bertoli, S. Casonato, S. Genovesi, A. Negro, M.C Sorgato. Human Doppel and prion protein share common membrane microdomains and internalization pathways. Int J Biochem Cell Biol 36, 2016-31, 2004.
  8. Peruffo A, Massimino ML, Ballarin C, Carmignoto G, Rota A, Cozzi B. Primary cultures from fetal bovine brain. Neuroreport., 15, 1719-22, 2004.
  9. Cereghetti GM, Negro A, Vinck E, Massimino ML, Sorgato MC, Van Doorslaer S. Copper(II) binding to the human Doppel protein may mark its functional diversity from the prion protein. J Biol Chem., 279,36497-503, 2004.
  10. Toni M, Massimino ML, Griffoni C, Salvato B, Tomasi V, Spisni E. Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells. FEBS Lett, 579, 741-4, 2005.
  11. Brunati AM, Deana R, Folda A, Massimino ML, Marin O, Ledro S, Pinna L, Donella-Deana A. Thrombin-induced Tyrosine Phosphorylation of HS1 in Human Platelets Is Sequentially Catalyzed by Syk and Lyn Tyrosine Kinases and Associated with the Cellular Migration of the Protein. J Biol Chem, 280, 21029-35, 2005.
  12. ML Massimino, J Ferrari, MC Sorgato, A Bertoli. Heterogeneous PrPC metabolism in skeletal muscle cells. FEBS Lett, 580, 878–884, 2006.
  13. Tibaldi E, Brunati AM, Massimino ML, Stringaro A, Colone M, Agostinelli E, Arancia G, Toninello A. Src-Tyrosine kinases are major agents in mitochondrial tyrosine phosphorylation. J Cell Biochem., 104, 840-849, 2008.
  14. Brunati AM, Tibaldi E, Carraro A, Gringeri E, D'Amico F Jr, Toninello A, Massimino ML, Pagano MA, Nalesso G, Cillo U. Cross-talk between PDGF and S1P signalling elucidates the inhibitory effect and potential antifibrotic action of the immunomodulator FTY720 in activated HSC-cultures. Biochim Biophys Acta, 1783, 347-359, 2008.
  15. Stella R, Massimino ML, Sandri M, Sorgato MC, Bertoli A. Cellular prion protein promotes regeneration of adult muscle tissue. Mol Cell Biol. 30, 4864-76, 2010.
  16. Stella R, Massimino ML, Sorgato MC, Bertoli A. Prion and TNFα: TAC(E)it agreement between the prion protein and cell signaling. Cell Cycle, 9, 4616-21, 2010
  17. Lazzari C, Peggion C, Stella R, Massimino ML, Lim D, Bertoli A, Sorgato MC. Cellular prion protein is implicated in the regulation of local Ca2+ movements in cerebellar granule neurons. J Neurochem. 116, 881-90, 2011. Dibattista M, Massimino ML, Maurya DK, Menini A, Bertoli A, Sorgato MC. The cellular prion protein is expressed in olfactory sensory neurons of adult mice but does not affect the early events of the olfactory transduction pathway. Chem Senses, 36, 791-7, 2011.
  18. Massimino ML, Redaelli M, Bertoli A, Sorgato MC, Mucignat-Caretta C. Altered behavioral aspects of aged mice lacking the cellular prion protein. Physiology and Behavior, 119, 86-91, 2013.
  19. Citta A, Schuh E, Mohr F, Folda A, Massimino ML, Bindoli A, Casini A, Rigobello MP. Fluorescent silver(i) and gold(i)-N-heterocyclic carbene complexes with cytotoxic properties: Mechanistic insights. Metallomics 5,1006-1015, 2013.
  20. Borgo C, Cesaro L, Salizzato V, Ruzzene M, Massimino ML, Pinna LA, Donella-Deana A. Aberrant signalling by protein kinase CK2 in imatinib-resistant chronic myeloid leukaemia cells: Biochemical evidence and therapeutic perspectives. Mol Oncol. 7, 1103-1115, 2013.
  21. Simonato M, Morbiato L, Zorzi V, Caccin P, Fernández J, Massimino ML, Polverino de Laureto P, Tonello F. Production in Escherichia coli, folding, purification and characterization of notexin with wild type sequence and with N-terminal and catalytic site mutations. Toxicon. 18, 11-20, 2014.
  22. Zanetti F, Carpi A, Menabò R, Giorgio M, Schulz R, Valen G, Baysa A, Massimino ML, Sorgato MC, Bertoli A, Di Lisa F. The cellular prion protein counteracts cardiac oxidative stress. Cardiovasc Res. 104, 93-102, 2014. doi: 10.1093/cvr/cvu194. Epub 2014 Aug 18
  23. Massimino ML, Peggion C, Loro F, Stella R, Megighian A, Scorzeto M, Blaauw B, Toniolo L, Sorgato MC, Reggiani C, Bertoli A. Age-dependent neuromuscular impairment in prion protein knock-out mice. Muscle Nerve 53, 269-79, 2016.
  24. De Mario A, Castellani A, Peggion C, Massimino ML, Lim D, Hill AF, Sorgato MC, Bertoli A. The prion protein constitutively controls neuronal store-operated Ca(2+) entry through Fyn kinase. Front Cell Neurosci. 2015 Oct 28;9:416. doi: 10.3389/fncel.2015.00416. eCollection 2015.
  25. Peggion C, Massimino ML, Biancotto G, Angeletti R, Reggiani C, Sorgato MC, Bertoli A, Stella R. Absolute quantification of myosin heavy chain isoforms by selected reaction monitoring can underscore skeletal muscle changes in a mouse model of amyotrophic lateral sclerosis. Anal Bioanal Chem. 2017 Mar;409(8):2143-2153. doi: 10.1007/s00216-016-0160-2. 2017
  26. Toni M, Massimino ML, De Mario A, Angiulli E, Spisni E. Metal Dyshomeostasis and Their Pathological Role in Prion and Prion-Like Diseases: The Basis for a Nutritional Approach. Front Neurosci. 2017 Jan 19;11:3. doi: 10.3389/fnins.2017.00003. eCollection 2017. Review
  27. De Mario A, Peggion C, Massimino ML, Viviani F, Castellani A, Giacomello M, Lim D, Bertoli A, Sorgato MC.The prion protein regulates glutamate-mediated Ca2+ entry and mitochondrial Ca2+ accumulation in neurons. J Cell Sci. 2017 Aug 15;130(16):2736-2746. doi: 10.1242/jcs.196972. 2017 
  28. Norante RP, Massimino ML, Lorenzon P, De Mario A, Peggion C, Vicario M, Albiero M, Sorgato MC, Lopreiato R, Bertoli A. Generation and validation of novel adeno-associated viral vectors for the analysis of Ca2+ homeostasis in motor neurons. Sci Rep. 2017 Jul 26;7(1):6521. doi: 10.1038/s41598-017-06919-0.
  29. Massimino ML, Simonato M, Spolaore B, Franchin C, Arrigoni G, Marin O, Monturiol-Gross L, Fernández J, Lomonte B, Tonello F. Cell surface nucleolin interacts with and internalizes Bothrops asper Lys49 phospholipase A2 and mediates its toxic activity. Sci Rep. 2018 Jul 13;8(1):10619. doi: 10.1038/s41598-018-28846-4.
  30. Peggion C, Stella R, Chemello F, Massimino ML, Arrigoni G, Cagnin S, Biancotto G, Franchin C, Sorgato MC, Bertoli A.The Prion Protein Regulates Synaptic Transmission by Controlling the Expression of Proteins Key to Synaptic Vesicle Recycling and Exocytosis. Mol Neurobiol. 2018 Aug 20. doi: 10.1007/s12035-018-1293-4.