Historically, our lab has been interested in calcium (Ca2+), its homeostasis, and its role in cell signalling, both in health and disease. One of the major topics of the lab over the last years has been the development and use of organelle targeted GFP-based fluorescent indicators for monitoring second messenger levels in living cells. These new tools, targeted to Endoplasmic reticulum (ER), Golgi apparatus, peroxisomes and mitochondria, have allowed for the first time to address the study of Ca2+ homeostasis in subcellular compartments for which until now only indirect information was available. In addition to Ca2+, much of the interest of our group is concentrated on cAMP probes. In this context, novel tools − again targeted to subcellular regions (plasma membrane, mitochondria, nucleus) − have been generated and characterized.

These methodologies are instrumental for addressing with novel approaches the role of second messenger heterogeneity in different physio-pathological conditions (i.e., the control of cardiac cell excitability, the role of second messengers in the formation of the sensory map in the olfactory bulb, the pathogenesis of Alzheimer’s disease) not only in cell lines or primary cell cultures, but also in more intact tissue preparations (acute slices) and in vivo.

 

5 recent publications

  1. Filadi R, Greotti E, Turacchio G, Luini A, Pozzan T, Pizzo P. Mitofusin 2 ablation increases endoplasmic reticulum-mitochondria coupling. Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):E2174-81. doi: 10.1073/pnas.1504880112. Epub 2015 Apr 13.
  2. Filadi R, Pozzan T. Generation and functions of second messengers microdomains.Cell Calcium. 2015 Mar 27. pii: S0143-4160(15)00061-5. doi: 10.1016/j.ceca.2015.03.007. [Epub ahead of print] Review.
  3. Lazzari C, Kipanyula MJ, Agostini M, Pozzan T and Fasolato C. Aβ42 oligomers selectively disrupt neuronal calcium release. Neurobiology of Aging 2015, 36, 877-885, DOI 10.1016/j.neurobiolaging.2014.10.020
  4. Pendin D, Greotti E, Filadi R, Pozzan T. Spying on organelle Ca2+ in living cells: the mitochondrial point of view.J Endocrinol Invest. 2014 Sep 23. [Epub ahead of print]
  5. Di Benedetto G, Pendin D, Greotti E, Pizzo P, Pozzan T. Ca2+ and cAMP cross-talk in mitochondria. J Physiol. 2014 Jan 15;592(Pt 2):305-12. doi: 10.1113 / jphysiol.2013.259135. Epub 2013 Jul 15. Review.

 

Grants

  1. University of Padova
  2. Cassa di Risparmio di Padova e Rovigo (CARIPARO) Foundation
  3. Italian Institute of Technology (IIT)
  4. Ministry of Education, University and Research (MIUR)
  5. National Research Council (CNR)