ER/Mitochondria contacts
Mitochondrial Ca2+ waves
3D mitochondrial structure

Description

The research activity focuses on the study of the mitochondrial calcium uniporter (MCU), the Ca2+ uptake pathway of mitochondria identified in 2011 by the group. The work includes the investigation of the structure/function relationship of MCU and its regulators, and of the role of MCU in the physiology and pathology of striated muscles, endocrine cells and neurons, both in cell cultures and in LoxP/CRE based transgenic mouse lines in which MCU is specifically ablated in a tissue.

Five recent publications

  • Vecellio Reane D, Vallese F, Checchetto V, Acquasaliente L, Butera G, De Filippis V, Szabò I, Zanotti G, Rizzuto R, Raffaello A. A MICU1 Splice Variant Confers High Sensitivity to the Mitochondrial Ca(2+) Uptake Machinery of Skeletal Muscle. Mol Cell. 2016 Nov 17;64(4):760-773. doi: 10.1016/j.molcel.2016.10.001. Epub 2016 Nov 3. PubMed PMID: 27818145.
  • Tosatto A, Sommaggio R, Kummerow C, Bentham RB, Blacker TS, Berecz T, Duchen MR, Rosato A, Bogeski I, Szabadkai G, Rizzuto R, Mammucari C. The mitochondrial calcium uniporter regulates breast cancer progression via HIF-1α. EMBO Mol Med. 2016 May 2;8(5):569-85. doi: 10.15252/emmm.201606255. Print 2016 May. PubMed PMID: 27138568; PubMed Central PMCID: PMC4864890.
  • Mammucari C, Gherardi G, Zamparo I, Raffaello A, Boncompagni S, Chemello F, Cagnin S, Braga A, Zanin S, Pallafacchina G, Zentilin L, Sandri M, De Stefani D, Protasi F, Lanfranchi G, Rizzuto R. The mitochondrial calcium uniporter controls skeletal muscle trophism in vivo. Cell Rep. 2015 Mar 3;10(8):1269-79. doi: 10.1016/j.celrep.2015.01.056. Epub 2015 Feb 26. PubMed PMID: 25732818; PubMed Central PMCID: PMC4351162.
  • De Stefani D, Raffaello A, Teardo E, Szabò I, Rizzuto R. A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. Nature. 2011 Jun 19;476(7360):336-40. doi: 10.1038/nature10230. PubMed PMID: 21685888;PubMed Central PMCID: PMC4141877.
  • Raffaello A, De Stefani D, Sabbadin D, Teardo E, Merli G, Picard A, Checchetto V, Moro S, Szabò I, Rizzuto R. The mitochondrial calcium uniporter is a multimer that can include a dominant-negative pore-forming subunit. EMBO J. 2013 Aug 28;32(17):2362-76. doi: 10.1038/emboj.2013.157. Epub 2013 Jul 30. PubMed PMID: 23900286; PubMed Central PMCID: PMC3771344.