Protein crystallography and cryoEM

Description

The group is involved in structural studies on different targets, from Helicobacter pylori proteins relevant for bacterial infection and pathogenesis, to cryoEM characterization of the calcium uniporter complex (MCU complex) and the plasma membrane calcium ATPase (PMCA).

At the functional level, two main lines of interest include the study of PMCA mutations linked to Cerebellar Ataxia and the role of Familial Parkinson Disease (PD)-related proteins in keeping mitochondrial wellness, cellular Ca2+ signaling and the contact sites between the endoplasmic reticulum (ER) and other intracellular organelles.

Five recent publications

  • Bercsenyi, N. Schmieg, J. B. Bryson, M. Wallace, P. Caccin, M. Golding, G. Zanotti, L. Greensmith, R. Nischt, G. Schiavo “Nidogens are therapeutic targets for the prevention of tetanus” Science, (2014) 346, 1118-1123 doi: 10.1126/science.1258138
  • Montecucco C. And Zanotti G., Botulinum neurotoxin A1 likes it double sweet, Nat. Struct. Mol. Biol. (2016) 23, 619-621
  • Md M. Shaik, L. Cendron, M. Salamina, M. Ruzzene And G. Zanotti “Helicobacter pylori receptor CeuE (HP1561) modulates its nickel affinity via organic metallophores” Mol. Microbiol (2014) 91, 724-735
  • Vicario M, Zanni G, Vallese F, Santorelli F, Grinzato A, Cieri D, Berto P, Frizzarin M, Lopreiato R, Zonta F, Ferro S, Sandre M, Marin O, Ruzzene M, Bertini E, Zanotti G, Brini M, Calì T, Carafoli E. A V1143F mutation in the neuronal-enriched isoform 2 of the PMCA pump is linked with ataxia. Neurobiol Dis. 2018 Jul;115:157-166. doi: 10.1016/j.nbd.2018.04.009. Epub 2018 Apr 12. PubMed PMID: 29655659.
  • Cieri D, Vicario M, Giacomello M, Vallese F, Filadi R, Wagner T, Pozzan T, Pizzo P, Scorrano L, Brini M, Calì T. SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition. Cell Death Differ. 2017 Dec 11. doi: 10.1038/s41418-017-0033-z.[Epub ahead of print] PubMed PMID: 29229997.
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