- Discovering Padova
- Il Bo
Historically, our lab has been interested in calcium (Ca2+), its homeostasis, and its role in cell signalling, both in health and disease. One of the major topics of the lab over the last years has been the development and use of organelle targeted GFP-based fluorescent indicators for monitoring second messenger levels in living cells. These new tools, targeted to Endoplasmic reticulum (ER), Golgi apparatus, peroxisomes and mitochondria, have allowed for the first time to address the study of Ca2+ homeostasis in subcellular compartments for which until now only indirect information was available. In addition to Ca2+, much of the interest of our group is concentrated on cAMP probes. In this context, novel tools − again targeted to subcellular regions (plasma membrane, mitochondria, nucleus) − have been generated and characterized.
These methodologies are instrumental for addressing with novel approaches the role of second messenger heterogeneity in different physio-pathological conditions (i.e., the control of cardiac cell excitability, the role of second messengers in the formation of the sensory map in the olfactory bulb, the pathogenesis of Alzheimer’s disease) not only in cell lines or primary cell cultures, but also in more intact tissue preparations (acute slices) and in vivo.