- Discovering Padova
- Il Bo
1969, High School Diploma at Liceo Classico "Arnaldo da Brescia", Brescia (Italy)
1974, Dr. Degree in Chemistry, University of Padua, cum laude,
1976-1978, Post-doctoral fellow, Department of Organic Chemistry, University of Padua
1979-1980, Visiting fellow, Laboratory of Molecular Biophysics, Oxford
1978-1983, Research Assistant, Dept. of Organic Chemistry, University of Padua
1983-2000, Associate Professor, Dept. of Organic Chemistry, University of Padua
2000-present, Full Professor, University of Padua
2008-2014, Director of the Graduate School in Biochemistry and Biotechnology
Since the beginning of its scientific career, Giuseppe Zanotti has worked at the determination of structure of macromolecules through X-ray diffraction. After a Dr. degree dissertation on bovine pancreatic Ribonuclease, he spent a year at the Laboratory of Molecular Biophysics in Oxford, where is took part in the structural determination of the structure of Glycogen Phosphorylase b. (Jenkins, et al. Phil. Trans. R. Soc. Lond., 1981, B293, 23-41; Stura, et al. J. Mol. Biol., 1983, 170, 529-565; Lorek, et al., Biochem. J., 1984, 218, 45-60). Back to Padua, he has been interested in proteins that bind and transport small hydrophobic molecules. In particular, he has determined the structure of proteins that bind retinoids, plasma and cellular Retinol Binding Proteins (Zanotti et al. J. Mol. Biol. 1993, 230, 613-624; Zanotti et al., J. Biol. Chem. 1993, 268, 10728-10738; Zanotti et al., J. Biol. Chem. 1993, 268, 24873-24879; Folli et al. J. Biol. Chem. 2002, 277, 41970-41977) and of Fatty Acid Binding Protein from human muscle (Zanotti et al., J. Biol. Chem., 1992, 267, 18541-18550). Subsequently he has been involved in structural determination of protein kinases, in particular of Ser/Thr kinase CK2. He has solved the structure of several complexes of catalytic subunit of CK2 with inhibitors (Sarno et al., Cell. Mol. Biol. Lett. 2001, 6, 480-481; Battistutta et al., Protein Sci. 2001, 10, 2200-2206; De Moliner, et al., J. Biol. Chem. 2003, 278, 1831-1836). A more recent field of interest is that of proteins from pathogenic bacteria: after determining the structure of some “dps-like” proteins from H. pylori, B. anthracis and B. burgdorferi, (Papinutto et al., J. Biol. Chem., 2002 277, 15093-15098; Zanotti et al., J. Mol. Biol., 2002, 323, 125-130) he is now involved in a project aimed at the systematic determination of the structures of proteins coded by the genes of the pathogenicity island of H. pylori, the bacterium that chronically infects the gastric mucosa of a large part of the human population. The structure of more than twenty proteins of the bacterium has been determined (Cendron et al., J. Mol. Biol. 2009, 386, 204-217; Cendron & Zanotti FEBS J. 2011, 1223-1231; Zanotti & L. Cendron World J. of Gastroenterology 2014, 20, 1402-1423; Shaik et al., Mol. Microbiol 2014, 91, 724-735).
The crystal structure of an integral membrane protein, the Calcium pump of the sarcoplasmic reticulum (SERCA) from bovine muscle, has also been determined (Sacchetto et al., J. Struct. Biol. 2012, 178, 38-44) and he is now trying to crystallize the calcium mitochondrial uniporter (MCU) and its regulatory partners.
Giuseppe Zanotti has also been interested in theoretical aspects of the phase problem in crystallography. He has published papers on phase extension using direct methods and Hilbert transforms (Zanotti et al., Acta Cryst. 1996, A52, 757-765).
More recently he has worked on the analysis and conformational aspects of the structure of globular proteins. He has proposed the theory that tensegrity could be a unifying motif of folding of globular proteins (Zanotti & Guerra FEBS letters 2003, 534, 7-10).
Giuseppe Zanotti has published 182 papers on international journals, 14 book chapters and some didactic books (3343 citations, H-index 32).
He has deposited 119 crystal structures at the Protein Data Bank.
The group in the last few years has been funded by grants from the University of Padua (Small scale structural genomics of Helicobacter pylori proteins) MIUR PRIN 2010-11 (Un approccio integrato per la comprensione dei determinanti strutturali e funzionali della patogenesi e persistenza di Helicobacter pylori), Futuro in ricerca 2013 (Structure and structural dynamics of Photosystem II supercomplex in higher plants upon exposure to different incident lights)